Moderate Hypothermia Has the Potential to Reveal the Dominant/Submissive Relationship in a Co-Culture System Consisting of Osteoblasts and Endothelial Cells

Microvessels in bone are indispensable for maintaining homeostasis based on a dynamic remodeling system. In cell-based tissue engineering, vascularization into the regenerative is key strategy to avoid hypoxia and necrosis around re-implanted tissues. Previous studies have shown that direct contact between osteoblasts endothelial cells stimulates differentiation of both cell types. However, no revealed dominant/submissive relationship. present study, we examined effect hypothermia monoculture co-culture assess which tightly coordinated osteogenesis angiogenesis As osteoblasts, exposure suppressed cellular proliferation, migration, differentiation. Evaluation behavior showed should not affect basic functions such as proliferation migration. Under conditions, osteogenic formation vessel-like angiogenic structures were by hypothermia, but spatial organization alkaline phosphatase-positive clusters, tend localize microvascular lumens, was altered. These data suggest attenuates heterotypic intercellular crosstalk robustly depends inhibit Taken together, this approach will provide new insights relationship engineering.